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Amersham Pharmacia Biotech Ltd multiphor ii apparatus
Multiphor Ii Apparatus, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+system/multiphor+ii+system/pm38002494-116-6-9
Average 90 stars, based on 1 article reviews
multiphor ii apparatus - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polyacrylamide Gel Electrophoresis:

Article Title: Characterizing the Host and Symbiont Proteomes in the Association between the Bobtail Squid, Euprymna scolopes , and the Bacterium, Vibrio fischeri
Article Snippet: .. 2-D PAGE was performed using the Amersham Pharmacia Biotech Multiphor II system as previously described . ..

Article Title: Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase.
Article Snippet: In a previous study the full-length open reading frame of the Arabian camel, Camelus dromedarius liver cytosolic glucose-6-phosphate dehydrogenase (G6PD) cDNA was determined using reverse transcription polymerase chain reaction.. The C. dromedarius cDNA was found to be 1545 nucleotides (accession number JN098421) that encodes a protein of 515 amino acids residues.. In the present study, Camelus dromedarius recombinant G6PD was heterologously overexpressed in Eschericia coli BL21 (DE3) pLysS and purified by immobilized metal affinity fast protein liquid chromatography (FPLC) in a single step.

Electrofocusing:

Article Title: Proteomic Analysis of Seed Dormancy in Arabidopsis
Article Snippet: .. Isoelectric focusing (IEF) of 200 μ g protein in reswelling buffer (8 m urea, 2% [w/v] CHAPS, 0.5% [v/v] immobilized pH gradient buffer 4–7, 20 m m dithiothreitol [DTT], and 0.01% [w/v] bromphenol blue) was run using 18-cm immobilized pH gradient (3–10 nonlinear) immobilized pH gradient strips on a Multiphor II system (Amersham-Pharmacia Biotech). ..

Article Title: Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase.
Article Snippet: In a previous study the full-length open reading frame of the Arabian camel, Camelus dromedarius liver cytosolic glucose-6-phosphate dehydrogenase (G6PD) cDNA was determined using reverse transcription polymerase chain reaction.. The C. dromedarius cDNA was found to be 1545 nucleotides (accession number JN098421) that encodes a protein of 515 amino acids residues.. In the present study, Camelus dromedarius recombinant G6PD was heterologously overexpressed in Eschericia coli BL21 (DE3) pLysS and purified by immobilized metal affinity fast protein liquid chromatography (FPLC) in a single step.

Article Title: Effects of high night temperature on lipid and protein compositions in tonoplasts isolated from Ananas comosus and Kalanchoë pinnata leaves
Article Snippet: Effects of high night temperature on the lipid and protein compositions in the tonoplasts isolated from the leaves of two Crassulacean acid metabolism (CAM) plants, Ananas comosus (pineapple) and Kalanchoë pinnata were studied.. The results showed that the phospholipids/protein ratios in the tonoplasts isolated from pineapple and K. pinnata leaves decreased from 1.82 to 1.21 and 2.63 to 1.50, respectively, as the night temperature increased from 20 to 37 oC.. Under high night temperature, relative amount of total unsaturated fatty acids in K. pinnata was increased by 6 %, which was mainly caused by increased C18:2 and C18:3, whereas unsaturated fatty acids, C18:2 and C18:3 in pineapple did not show significant change.

Recombinant:

Article Title: Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase.
Article Snippet: In a previous study the full-length open reading frame of the Arabian camel, Camelus dromedarius liver cytosolic glucose-6-phosphate dehydrogenase (G6PD) cDNA was determined using reverse transcription polymerase chain reaction.. The C. dromedarius cDNA was found to be 1545 nucleotides (accession number JN098421) that encodes a protein of 515 amino acids residues.. In the present study, Camelus dromedarius recombinant G6PD was heterologously overexpressed in Eschericia coli BL21 (DE3) pLysS and purified by immobilized metal affinity fast protein liquid chromatography (FPLC) in a single step.

Electrophoresis:

Article Title: Brood parasitism, female condition and clutch reduction in the common eider Somateria mollisima
Article Snippet: Conspecific brood parasitism (CBP) is an alternative reproductive tactic found in many animals with parental care.. Parasitizing females lay eggs in the nests of other females (hosts) of the same species, which incubate and raise both their own and the foreign offspring.. The causes and consequences of CBP are debated.

Article Title: Host?parasite kinship in a female-philopatric bird population: evidence from relatedness trend analysis
Article Snippet: Conspecific brood parasitism (CBP), an alternative reproductive tactic where some females lay eggs in the nests of other females of the same species, occurs in many animals with egg care.. It is particularly common in waterfowl, for reasons that are debated.. Many waterfowl females nest near their birthplace, making it likely that some local females are relatives.

Protein Extraction:

Article Title: Effects of high night temperature on lipid and protein compositions in tonoplasts isolated from Ananas comosus and Kalanchoë pinnata leaves
Article Snippet: Effects of high night temperature on the lipid and protein compositions in the tonoplasts isolated from the leaves of two Crassulacean acid metabolism (CAM) plants, Ananas comosus (pineapple) and Kalanchoë pinnata were studied.. The results showed that the phospholipids/protein ratios in the tonoplasts isolated from pineapple and K. pinnata leaves decreased from 1.82 to 1.21 and 2.63 to 1.50, respectively, as the night temperature increased from 20 to 37 oC.. Under high night temperature, relative amount of total unsaturated fatty acids in K. pinnata was increased by 6 %, which was mainly caused by increased C18:2 and C18:3, whereas unsaturated fatty acids, C18:2 and C18:3 in pineapple did not show significant change.



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Specification of the Relevant Instrumental Techniques for LF Characterization

Journal: Journal of Agricultural and Food Chemistry

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

doi: 10.1021/acs.jafc.3c06887

Figure Lengend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

Article Snippet: , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) .

Techniques: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection